Patch Clamp Fluorometry in Outside-Out Patches for Ligand-Gated Ion Channels
نویسندگان
چکیده
منابع مشابه
Gating Rearrangements in Cyclic Nucleotide-Gated Channels Revealed by Patch-Clamp Fluorometry
Site-specific fluorescence recordings have shown great promise in understanding conformational changes in signaling proteins. The reported applications on ion channels have been limited to extracellular sites in whole oocyte preparations. We are now able to directly monitor gating movements of the intracellular domains of cyclic nucleotide-gated channels using simultaneous site-specific fluores...
متن کاملPatch fluorometry: shedding new light on ion channels.
Patch fluorometry has emerged as a new approach to the study of the structure-function relationship in membrane-embedded functional ion channels. Simultaneous fluorescent and electrical recordings are achieved from a small number of channels in a cell-free membrane patch, yielding high recording sensitivities. Further improvement of this approach should permit direct observation of the gating m...
متن کاملAllosteric coupling in ligand-gated ion channels
What does " allosteric " mean? In the context of receptors, the word allosteric is now widely used. It is, perhaps, not helpful for clarity of thought that different authors often use it to mean somewhat different things (Colquhoun, 1998). At one extreme, the term " allosteric antagonist " can often be translated as " we have got an antagonist and we are not sure what it does, but it appears no...
متن کاملDynamics of Ca2+-Calmodulin–dependent Inhibition of Rod Cyclic Nucleotide-gated Channels Measured by Patch-clamp Fluorometry
Cyclic nucleotide-gated (CNG) ion channels mediate cellular responses to sensory stimuli. In vertebrate photoreceptors, CNG channels respond to the light-induced decrease in cGMP by closing an ion-conducting pore that is permeable to cations, including Ca(2+) ions. Rod CNG channels are directly inhibited by Ca(2+)-calmodulin (Ca(2+)/CaM), but the physiological role of this modulation is unknown...
متن کاملPatch clamp recording of ion channels expressed in Xenopus oocytes.
Since its development by Sakmann and Neher (1, 2), the patch clamp has become established as an extremely useful technique for electrophysiological measurement of single or multiple ion channels in cells. This technique can be applied to ion channels in both their native environment and expressed in heterologous cells, such as oocytes harvested from the African clawed frog, Xenopus laevis. Here...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2016
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2015.11.3215